Localization and Composition of Fructans in Stem and Rhizome of Agave tequilana Weber var. azul.

ABSTRACT: Methodology combining mass spectrometry imaging (MSI) with ion mobility separation (IMS) has emerged as a biological imaging technique due to its versatility, sensitivity and label-free approach. This technique has been shown to separate isomeric compounds such as lipids, amino acids, carboxylic acids and carbohydrates. This report describes mass spectrometry imaging in combination with traveling-wave ion mobility separation and matrix-assisted laser desorption/ionization (MALDI). Positive ionization mode was used to locate fructans on tissue printed sections of Agave rhizome and stem tissue and distinguished fructan isoforms. Here we show the location of fructans ranging from DP3 to DP17 to be differentially abundant across the stem tissue and for the first time, experimental colli

SUBMITTER: Perez-Lopez AV 

PROVIDER: S-EPMC7855178 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature