Measuring DNA mechanics on the genome scale.

ABSTRACT: Mechanical deformations of DNA such as bending are ubiquitous and have been implicated in diverse cellular functions¹. However, the lack of high-throughput tools to measure the mechanical properties of DNA has limited our understanding of how DNA mechanics influence chromatin transactions across the genome. Here we develop 'loop-seq'-a high-throughput assay to measure the propensity for DNA looping-and determine the intrinsic cyclizabilities of 270,806 50-base-pair DNA fragments that span Saccharomyces cerevisiae chromosome V, other genomic regions, and random sequences. We found sequence-encoded regions of unusually low bendability within nucleosome-depleted regions upstream of transcription start sites (TSSs). Low bendability of linker DNA inhibits nucleosome sliding into the

SUBMITTER: Basu A 

PROVIDER: S-EPMC7855230 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature