Extraction optimization for combined metabolomics, peptidomics, and proteomics analysis of gut microbiota samples.

ABSTRACT: Multiomic studies are increasingly performed to gain a deeper understanding of molecular processes occurring in a biological system, such as the complex microbial communities (i.e., microbiota) that reside the distal gut. While a combination of metabolomics and proteomics is more commonly used, multiomics studies including peptidomcis characterization are less frequently undertaken. Here, we investigated three different extraction methods, chosen for their previous use in extracting metabolites, peptides, and proteins, and compared their ability to perform metabolomic, peptidomic, and proteomic analysis of mouse cecum content. The methanol/chloroform/water extraction performed the best for metabolomic and peptidomic analysis as it detected the largest number of small molecules and identifi

SUBMITTER: Keller C 

PROVIDER: S-EPMC7855350 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature