Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation.

ABSTRACT: Directed differentiation of human pluripotent stem cells to kidney organoids brings the prospect of drug screening, disease modelling and the generation of tissue for renal replacement. Currently, these applications are hampered by organoid variability, nephron immaturity, low throughput and limited scale. Here, we apply extrusion-based three-dimensional cellular bioprinting to deliver rapid and high-throughput generation of kidney organoids with highly reproducible cell number and viability. We demonstrate that manual organoid generation can be replaced by 6- or 96-well organoid bioprinting and evaluate the relative toxicity of aminoglycosides as a proof of concept for drug testing. In addition, three-dimensional bioprinting enables precise manipulation of biophysical properties, includin

SUBMITTER: Lawlor KT 

PROVIDER: S-EPMC7855371 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature