Proteomic, biomechanical and functional analyses define neutrophil heterogeneity in systemic lupus erythematosus.

ABSTRACT:

Objectives

Low-density granulocytes (LDGs) are a distinct subset of proinflammatory and vasculopathic neutrophils expanded in systemic lupus erythematosus (SLE). Neutrophil trafficking and immune function are intimately linked to cellular biophysical properties. This study used proteomic, biomechanical and functional analyses to further define neutrophil heterogeneity in the context of SLE.

Methods

Proteomic/phosphoproteomic analyses were performed in healthy control (HC) normal density neutrophils (NDNs), SLE NDNs and autologous SLE LDGs. The biophysical properties of these neutrophil subsets were analysed by real-time deformability cytometry and lattice light-sheet microscopy. A two-dimensional endothelial flow system and a three-dimensional microfluidic microvasculature m

SUBMITTER: Bashant KR 

PROVIDER: S-EPMC7855438 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature