Design of biologically active binary protein 2D materials.

ABSTRACT: Ordered two-dimensional arrays such as S-layers^1,2 and designed analogues^3-5 have intrigued bioengineers^6,7, but with the exception of a single lattice formed with flexible linkers⁸, they are constituted from just one protein component. Materials composed of two components have considerable potential advantages for modulating assembly dynamics and incorporating more complex functionality^9-12. Here we describe a computational method to generate co-assembling binary layers by designing rigid interfaces between pairs of dihedral protein building blocks, and use it to design a p6m lattice. The designed array components are soluble at millimolar concentrations, but when combined at nanomolar concentrations, they rapidly assemble into nearly

SUBMITTER: Ben-Sasson AJ 

PROVIDER: S-EPMC7855610 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature