Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins.

ABSTRACT:

Background

During development, all retinal cell types arise from retinal progenitor cells (RPCs) in a step-wise fashion. Atoh7 and Pou4f2 mark, and function in, two phases of retinal ganglion cell (RGC) genesis; Atoh7 functions in a subpopulation of RPCs to render them competent for the RGC fate, whereas Pou4f2 participates in RGC fate specification and RGC differentiation. Despite extensive research on their roles, the properties of the two phases represented by these two factors have not been well studied, likely due to the retinal cellular heterogeneity.

Results

In this report, we describe two novel knock-in mouse alleles, Atoh7^{zsGreenCreERT2} and Pou4f2^FlagtdTomato , which labeled retinal cells in the two phases of RGC development by fluorescent prot

SUBMITTER: Ge Y 

PROVIDER: S-EPMC7855626 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature