Apoptotic Neuron-Derived Histone Amyloid Fibrils Induce α-Synuclein Aggregation.

ABSTRACT: Cell-to-cell transfer of α-synuclein (αS) is increasingly thought to play an important role in propagation of αS pathology, but mechanisms responsible for formation of initial αS seeds and factors facilitating their propagation remain unclear. We previously demonstrated that αS aggregates are formed rapidly in apoptotic neurons and that interaction between cytoplasmic αS and proaggregant nuclear factors generates seed-competent αS. We also provided initial evidence that histones have proaggregant properties. Since histones are released from cells undergoing apoptosis or cell stress, we hypothesized that internalization of histones into αS expressing cells could lead to intracellular αS aggregation. Here using mCherry-tagged histone, we show that nuclear extracts from apoptotic cells can in

SUBMITTER: Jiang P 

PROVIDER: S-EPMC7855663 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature