Collagen Organization in Relation to Ductal Carcinoma In Situ Pathology and Outcomes.

ABSTRACT:

Background

There is widespread interest in discriminating indolent from aggressive ductal carcinoma in situ (DCIS). We sought to evaluate collagen organization in the DCIS tumor microenvironment in relation to pathologic characteristics and patient outcomes.

Methods

We retrieved fixed tissue specimens for 90 DCIS cases within the population-based Vermont DCIS Cohort. We imaged collagen fibers within 75 μm of the tumor/stromal boundary on hematoxylin and eosin-stained slides using multiphoton microscopy with second-harmonic generation. Automated software quantified collagen fiber length, width, straightness, density, alignment, and angle to the tumor/stroma boundary. Factor analysis identified linear combinations of collagen fiber features representing composite attrib

SUBMITTER: Sprague BL 

PROVIDER: S-EPMC7855820 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature