A photo-crosslinkable cartilage-derived extracellular matrix bioink for auricular cartilage tissue engineering.

ABSTRACT: Three-dimensional (3D) bioprinting of patient-specific auricular cartilage constructs could aid in the reconstruction process of traumatically injured or congenitally deformed ear cartilage. To achieve this, a hydrogel-based bioink is required that recapitulates the complex cartilage microenvironment. Tissue-derived decellularized extracellular matrix (dECM)-based hydrogels have been used as bioinks for cell-based 3D bioprinting because they contain tissue-specific ECM components that play a vital role in cell adhesion, growth, and differentiation. In this study, porcine auricular cartilage tissues were isolated and decellularized, and the decellularized cartilage tissues were characterized by histology, biochemical assay, and proteomics. This cartilage-derived dECM (cdECM) was subsequentl

SUBMITTER: Visscher DO 

PROVIDER: S-EPMC7855948 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature