A fluorescence sandwich immunoassay for the real-time continuous detection of glucose and insulin in live animals.

ABSTRACT: Biosensors that continuously measure circulating biomolecules in real time could provide insights into the health status of patients and their response to therapeutics. But biosensors for the continuous real-time monitoring of analytes in vivo have only reached nanomolar sensitivity and can measure only a handful of molecules, such as glucose and blood oxygen. Here we show that multiple analytes can be continuously and simultaneously measured with picomolar sensitivity and sub-second resolution via the integration of aptamers and antibodies into a bead-based fluorescence sandwich immunoassay implemented in a custom microfluidic chip. After an incubation time of 30 s, bead fluorescence is measured using a high-speed camera under spatially multiplexed two-colour laser illumination. We used t

SUBMITTER: Poudineh M 

PROVIDER: S-EPMC7856282 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature