Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary.

ABSTRACT: Neuropeptides and peptide hormones are important cell-cell signaling molecules that mediate many physiological processes. Unlike classic neurotransmitters, peptides undergo cell-type-specific post-translational modifications that affect their biological activity. To enable the identification of the peptide repertoire of a genetically defined cell type, we generated mice with a conditional disruption of the gene for carboxypeptidase E (Cpe), an essential neuropeptide-processing enzyme. The loss of Cpe leads to accumulation of neuropeptide precursors containing C-terminal basic residues, which serve as tags for affinity purification. The purified peptides are subsequently identified using quantitative peptidomics, thereby revealing the specific forms of neuropeptides in cells with the disrup

SUBMITTER: Fricker LD 

PROVIDER: S-EPMC7856292 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature