Nox4 mediates skeletal muscle metabolic responses to exercise.

ABSTRACT:

Objective

The immediate signals that couple exercise to metabolic adaptations are incompletely understood. Nicotinamide adenine dinucleotide phosphate oxidase 4 (Nox4) produces reactive oxygen species (ROS) and plays a significant role in metabolic and vascular adaptation during stress conditions. Our objective was to determine the role of Nox4 in exercise-induced skeletal muscle metabolism.

Methods

Mice were subjected to acute exercise to assess their immediate responses. mRNA and protein expression responses to Nox4 and hydrogen peroxide (H₂O₂) were measured by qPCR and immunoblotting. Functional metabolic flux was measured via ex vivo fatty acid and glucose oxidation assays using ¹⁴C-labeled palmitate and glucose, respectively. A chronic e

SUBMITTER: Specht KS 

PROVIDER: S-EPMC7856463 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature