Rapid and Ultrasensitive Detection of Mutations and Genes Relevant to Antimicrobial Resistance in Bacteria.

ABSTRACT: The worldwide emergence of multidrug-resistant (MDR) bacteria is associated with significant morbidity, mortality, and healthcare costs. Rapid and accurate diagnostic methods to detect antibiotic resistance are critical for antibiotic stewardship and infection control measurements. Here a cantilever nanosensor-based diagnostic assay is shown to detect single nucleotide polymorphisms (SNPs) and genes associated with antibiotic resistance in Gram negative (Pseudomonas aeruginosa) and positive (Enterococcus faecium) bacteria, representing frequent causes for MDR infections. Highly specific RNA capture probes for SNPs (ampR^D135G or ampR^G154R ) or resistance genes (vanA, vanB, and vanD) allow to detect the binding of bacteri

SUBMITTER: Huber F 

PROVIDER: S-EPMC7857129 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature