A Highly Reproducible Micro U-Well Array Plate Facilitating High-Throughput Tumor Spheroid Culture and Drug Assessment.

ABSTRACT: 3D multicellular tumor spheroids (MCTSs) have recently emerged as a landmark for cancer research due to their inherent traits that are physiologically relevant to primary tumor microenvironments. A facile approach-laser-ablated micro U-wells-has been widely adopted in the past decade. However, the differentiation of microwell uniformities and the construction of arrays have all remained elusive. Herein, an improved laser-ablated microwell array technique is proposed that can not only achieve arrayed MCTSs with identical sizes but can also perform high-throughput drug assessments in situ. Three critical laser ablation parameters, including frequency, duty cycle, and pulse number, are investigated to generate microwells flexibly with a range from 170 to 400 μm. The choice of microwells is op

SUBMITTER: Wu KW 

PROVIDER: S-EPMC7857131 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature