Alternative pathways utilize or circumvent putrescine for biosynthesis of putrescine-containing rhizoferrin.

ABSTRACT: The siderophore rhizoferrin (N¹,N⁴-dicitrylputrescine) is produced in fungi and bacteria to scavenge iron. Putrescine-producing bacterium Ralstonia pickettii synthesizes rhizoferrin and encodes a single nonribosomal peptide synthetase-independent siderophore (NIS) synthetase. From biosynthetic logic, we hypothesized that this single enzyme is sufficient for rhizoferrin biosynthesis. We confirmed this by expression of R. pickettii NIS synthetase in Escherichia coli, resulting in rhizoferrin production. This was further confirmed in vitro using the recombinant NIS synthetase, synthesizing rhizoferrin from putrescine and citrate. Heterologous expression of homologous lbtA from Legionella pneumophila, required for rhizoferrin biosynthesis in that species, produced siderop

SUBMITTER: Li B 

PROVIDER: S-EPMC7857480 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature