SPOCK, an R based package for high-throughput analysis of growth rate, survival, and chronological lifespan in yeast.

ABSTRACT: Plate reader-based methods for high-throughput measurement of growth rate, cellular survival, and chronological lifespan are a compelling addition to the already powerful toolbox of budding yeast Saccharomyces cerevisiae genetics. These methods have overcome many of the limits of traditional yeast biology techniques, but also present a new bottleneck at the point of data-analysis. Herein, we describe SPOCK (Survival Percentage and Outgrowth Collection Kit), an R-based package for the analysis of data created by high-throughput plate reader based methods. This package allows for the determination of chronological lifespan, cellular growth rate, and survival in an efficient, robust, and reproducible fashion.

SUBMITTER: Small EM 

PROVIDER: S-EPMC7857483 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature