Evaluation of CRISPR Diversity in the Human Skin Microbiome for Personal Identification.

ABSTRACT: The highly personalized human skin microbiome may serve as a viable marker in personal identification. Amplicon sequencing resolution using 16S rRNA cannot identify bacterial communities sufficiently to discriminate between individuals. Thus, novel higher-resolution genetic markers are required for forensic purposes. The clustered regularly interspaced short palindromic repeats (CRISPRs) are prokaryotic genetic elements that can provide a history of infections encountered by the bacteria. The sequencing of CRISPR spacers may provide phylogenetic information with higher resolution than other markers. However, using spacer sequencing for discrimination of personal skin microbiome is difficult due to limited information on CRISPRs in human skin microbiomes. It remains unclear whether personal

SUBMITTER: Toyomane K 

PROVIDER: S-EPMC7857535 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature