Cell-type specific knockout of peptidylglycine α-amidating monooxygenase reveals specific behavioral roles in excitatory forebrain neurons and cardiomyocytes.

ABSTRACT: Neuropeptides and peptide hormones play a crucial role in integrating the many factors that affect physiologic and cognitive processes. The potency of many of these peptides requires an amidated amino acid at the C-terminus; a single enzyme, peptidylglycine α-amidating monooxygenase (PAM), catalyzes this modification. Anxiety-like behavior is known to be altered in mice with a single functional Pam allele (Pam^+/- ) and in mice unable to express Pam in excitatory forebrain neurons (Pam^Emx1-cKO/cKO ) or in cardiomyocytes (Pam^Myh6-cKO/cKO ). Examination of PAM-positive and glutamic acid decarboxylase 67 (GAD)-positive cells in the amygdala of Pam^Emx1-cKO/cKO mice demonstrated the absence of PAM in pyramidal neurons and its continued presence in GAD-

SUBMITTER: Powers KG 

PROVIDER: S-EPMC7858240 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature