Localisation of clozapine during experimental autoimmune encephalomyelitis and its impact on dopamine and its receptors.

ABSTRACT: Multiple sclerosis is a disease characterised by axonal demyelination in the central nervous system (CNS). The atypical antipsychotic drug clozapine attenuates experimental autoimmune encephalomyelitis (EAE), a mouse model used to study multiple sclerosis, but the precise mechanism is unknown and could include both peripheral and CNS-mediated effects. To better understand where clozapine exerts its protective effects, we investigated the tissue distribution and localisation of clozapine using matrix-assisted laser desorption ionization imaging mass spectrometry and liquid chromatography-mass spectrometry. We found that clozapine was detectable in the brain and enriched in specific brain regions (cortex, thalamus and olfactory bulb), but the distribution was not altered by EAE. Furthermore,

SUBMITTER: Robichon K 

PROVIDER: S-EPMC7858600 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature