Bactericidal Permeability Increasing Protein Deficiency Aggravates Acute Colitis in Mice by Increasing the Serum Levels of Lipopolysaccharide.

ABSTRACT:

Objective

The objective of this study was to understand the role of bactericidal permeability increasing protein (BPI) in the pathogenesis of experimental murine colitis.

Methods

We used the Cre-LoxP system to generate BPI knockout (BPI KO) mice. Acute colitis was induced in BPI KO mice and wild-type (WT) mice by subjecting the mice to 5% dextran sulfate sodium (DSS). Mice were observed for symptoms of experimental colitis. The survival of BPI KO mice to infection with Acinetobacter baumannii, a gram-negative bacterium, was also assessed.

Results

Southern blot, RT-PCR, and western blot results showed that the 2^nd and 3^rd exons of the murine Bpi gene were knocked out systemically, confirming successful construction of the BPI KO mous

SUBMITTER: Kong Q 

PROVIDER: S-EPMC7858664 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature