Activation of the Nlrp3 Inflammasome Contributes to Shiga Toxin-Induced Hemolytic Uremic Syndrome in a Mouse Model.

ABSTRACT:

Objective

To explore the role of the Nlrp3 inflammasome activation in the development of hemolytic uremic syndrome (HUS) induced by Stx2 and evaluate the efficacy of small molecule Nlrp3 inhibitors in preventing the HUS.

Methods

Peritoneal macrophages (PMs) isolated from wild-type (WT) C57BL/6J mice and gene knockout mice (Nlrc4^-/-, Aim2^-/-, and Nlrp3^-/-) were treated with Stx2 in vitro and their IL-1β releases were measured. WT mice and Nlrp3^-/- mice were also treated with Stx2 in vivo by injection, and the biochemical indices (serum IL-1β, creatinine [CRE] and blood urea nitrogen [BUN]), renal injury, and animal survival were compared. To evaluate the effect of the Nlrp3 inhibitors in pre

SUBMITTER: Song L 

PROVIDER: S-EPMC7859089 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature