Vancomycin Wrap for Anterior Cruciate Ligament Surgery: Molecular Insights.

ABSTRACT:

Background

The use of the vancomycin wrap to pretreat the hamstring graft in anterior cruciate ligament reconstruction (ACLR) has grown in popularity since it was first described in 2012 and has significantly reduced rates of postoperative infection. However, it remains unknown if this antibiotic treatment affects the molecular composition of the graft.

Purpose

To establish whether treatment with vancomycin at 5 mg/mL, the most commonly used concentration, alters the molecular function of the hamstring graft in ACLR.

Study design

Controlled laboratory study.

Methods

Surplus hamstring tendon collected after routine ACLR surgery was used for in vitro cell culture and ex vivo tissue experiments. Vancomycin was used at 5 mg/mL in RPMI or saline diluent to treat cel

SUBMITTER: Atherton CM 

PROVIDER: S-EPMC7859666 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature