Systemic Immunomodulatory Effects of Combinatorial Treatment of Thalidomide and Dexamethasone on T Cells and Other Immune Cells.

ABSTRACT:

Purpose

In organ transplantation, the need for immune modulation rather than immune suppression has been emphasized. In this study, we investigated whether combinatorial treatments of with thalidomide (TM) and dexamethasone (DX) might be new approaches to induce systemic immunomodulation on T cells and other immune cells that regulate the expression of co-inhibitory molecules.

Materials and methods

Naïve splenic T cells from C57BL/6 mice were sort-purified and cultured in vitro for CD4⁺ T cell proliferation and regulatory T cell (Treg) conversion in the presence of TM or/and DX. Expression of cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) and programmed death-1 (PD-1) in proliferated and converted T cells was quantified by flow cytometry. We also quantified

SUBMITTER: Kim EJ 

PROVIDER: S-EPMC7859687 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature