MiR-126-3p contributes to sorafenib resistance in hepatocellular carcinoma via downregulating SPRED1.

ABSTRACT:

Background

Sorafenib can prolong the survival of patients with advanced hepatocellular carcinoma (HCC). However, drug resistance remains the main obstacle to improving its efficiency. This study aimed to explore the likely molecular mechanism of sorafenib resistance.

Methods

Differentially expressed microRNAs (miRNAs) related to sorafenib response were analyzed with the Limma package in R software. The expression levels of miR-126-3p and sprouty-related EVH1 domain-containing protein 1 (SPRED1) in HCC cells were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Cell viability and proliferation were detected with Cell Counting Kit-8 (CCK-8), EdU proliferation, and clone formation assays. Transwell assays were performed to measure cell migrati

SUBMITTER: Tan W 

PROVIDER: S-EPMC7859776 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature