Electrospun fibers enhanced the paracrine signaling of mesenchymal stem cells for cartilage regeneration.

ABSTRACT:

Background

Secretome profiles of mesenchymal stem cells (MSCs) are reflective of their local microenvironments. These biologically active factors exert an impact on the surrounding cells, eliciting regenerative responses that create an opportunity for exploiting MSCs towards a cell-free therapy for cartilage regeneration. The conventional method of culturing MSCs on a tissue culture plate (TCP) does not provide the physiological microenvironment for optimum secretome production. In this study, we explored the potential of electrospun fiber sheets with specific orientation in influencing the MSC secretome production and its therapeutic value in repairing cartilage.

Methods

Conditioned media (CM) were generated from MSCs cultured either on TCP or electrospun fiber sheets of di

SUBMITTER: Kadir ND 

PROVIDER: S-EPMC7860031 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature