Phage-Display-Derived Peptide Specific to Carbamylated Protein.

ABSTRACT: Protein carbamylation has been linked with diseases commonly associated with patients with reduced kidney function. Carbamylated human serum albumin (cHSA), which has been proven to be nephrotoxic and associated with heart failure for chronic kidney disease (CKD) patients, was chosen for our study. Through phage display against cHSA, one specific peptide sequence (cH2-p1) was identified with higher selectivity toward cHSA over native HSA. The cH2-p1 peptide was synthesized, and its target binding was analyzed through isothermal titration calorimetry (ITC). The result showed that cH2-p1 was able to bind cHSA of different levels of carbamylation with a similar dissociation constant of ∼1.0 × 10^-4 M. This peptide also showed a binding specificity to carbamylated fibrinogen (cFgn),

SUBMITTER: Ma Y 

PROVIDER: S-EPMC7860060 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature