Long non-coding RNA H19 expression and functional polymorphism rs217727 are linked to increased ischemic stroke risk.

ABSTRACT:

Background

Efforts to identify potential biomarkers for the diagnosis of ischemic stroke (IS) are valuable. The H19 gene plays a functional role in increasing the prevalence of IS risk factors. We evaluated the correlation between H19 rs217727 polymorphism and the expression level of H19 lncRNA with susceptibility to IS among the Iranian population.

Methods

Blood samples were collected from IS patients (n = 114) and controls (n = 114). We concentrated on the expression pattern of H19 at different time points (i.e., 0-24, 24-48, and 48-72 h after stroke). The tetra-amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) method was applied for DNA genotyping. We used the quantitative real-time PCR to evaluate H19 expression levels. We used the receiver

SUBMITTER: Rezaei M 

PROVIDER: S-EPMC7860182 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature