Reverse-Phase Protein Array: Technology, Application, Data Processing, and Integration.

ABSTRACT: Reverse-phase protein array (RPPA) is a high-throughput antibody-based targeted proteomics platform that can quantify hundreds of proteins in thousands of samples derived from tissue or cell lysates, serum, plasma, or other body fluids. Protein samples are robotically arrayed as microspots on nitrocellulose-coated glass slides. Each slide is probed with a specific antibody that can detect levels of total protein expression or post-translational modifications, such as phosphorylation as a measure of protein activity. Here we describe workflow protocols and software tools that we have developed and optimized for RPPA in a core facility setting that includes sample preparation, microarray mapping and printing of protein samples, antibody labeling, slide scanning, image analysis, data normaliz

SUBMITTER: Coarfa C 

PROVIDER: S-EPMC7861052 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature