Enhanced Collision Induced Unfolding and Electron Capture Dissociation of Native-like Protein Ions.

ABSTRACT: Native ion mobility-mass spectrometry (IM-MS) is capable of revealing much that remains unknown within the structural proteome, promising such information on refractory protein targets. Here, we report the development of a unique drift tube IM-MS (DTIM-MS) platform, which combines high-energy source optics for improved collision induced unfolding (CIU) experiments and an electromagnetostatic cell for electron capture dissociation (ECD). We measured a series of high precision collision cross section (CCS) values for protein and protein complex ions ranging from 6-1600 kDa, exhibiting an average relative standard deviation (RSD) of 0.43 ± 0.20%. Furthermore, we compare our CCS results to previously reported DTIM values, finding strong agreement across similarly configured instrumentation (av

SUBMITTER: Gadkari VV 

PROVIDER: S-EPMC7861131 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature