Cell-free tumour DNA analysis detects copy number alterations in gastro-oesophageal cancer patients.

ABSTRACT:

Background

Analysis of cell-free tumour DNA, a liquid biopsy, is a promising biomarker for cancer. We have performed a proof-of principle study to test the applicability in the clinical setting, analysing copy number alterations (CNAs) in plasma and tumour tissue from 44 patients with gastro-oesophageal cancer.

Methods

DNA was isolated from blood plasma and a tissue sample from each patient. Array-CGH was applied to the tissue DNA. The cell-free plasma DNA was sequenced by low-coverage whole-genome sequencing using a clinical pipeline for non-invasive prenatal testing. WISECONDOR and ichorCNA, two bioinformatic tools, were used to process the output data and were compared to each other.

Results

Cancer-associated CNAs could be seen in 59% (26/44) of the tissue biopsies

SUBMITTER: Wallander K 

PROVIDER: S-EPMC7861431 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature