Dissecting the role of TP53 alterations in del(11q) chronic lymphocytic leukemia.

ABSTRACT:

Background

Several genetic alterations have been identified as driver events in chronic lymphocytic leukemia (CLL) pathogenesis and oncogenic evolution. Concurrent driver alterations usually coexist within the same tumoral clone, but how the cooperation of multiple genomic abnormalities contributes to disease progression remains poorly understood. Specifically, the biological and clinical consequences of concurrent high-risk alterations such as del(11q)/ATM-mutations and del(17p)/TP53-mutations have not been established.

Methods

We integrated next-generation sequencing (NGS) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 techniques to characterize the in vitro and in vivo effects of concurrent monoallelic or biallelic ATM and/or TP53 alterations

SUBMITTER: Quijada-Alamo M 

PROVIDER: S-EPMC7862176 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature