Histone modifications form a cell-type-specific chromosomal bar code that persists through the cell cycle.

ABSTRACT: Chromatin configuration influences gene expression in eukaryotes at multiple levels, from individual nucleosomes to chromatin domains several Mb long. Post-translational modifications (PTM) of core histones seem to be involved in chromatin structural transitions, but how remains unclear. To explore this, we used ChIP-seq and two cell types, HeLa and lymphoblastoid (LCL), to define how changes in chromatin packaging through the cell cycle influence the distributions of three transcription-associated histone modifications, H3K9ac, H3K4me3 and H3K27me3. We show that chromosome regions (bands) of 10-50 Mb, detectable by immunofluorescence microscopy of metaphase (M) chromosomes, are also present in G₁ and G₂. They comprise 1-5 Mb sub-bands that differ between HeLa and LCL

SUBMITTER: Halsall JA 

PROVIDER: S-EPMC7862352 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature