Corneal Repair with Adhesive Cell Sheets of Fetal Cartilage-Derived Stem Cells.

ABSTRACT:

Background

Corneal scarring or disease may lead to severe corneal opacification and consequently, severe loss of vision due to the complete loss of corneal epithelial cells. We studied the use of epithelial cell sheets differentiated from fetal cartilage-derived stem cells (FCSC) to resurface damaged cornea.

Methods

The FCSC were isolated from the femoral head of immature cartilage tissue. The ability of the FCSCs to differentiate into corneal epithelial cells was evaluated using differentiation media at 2 days and 7 days post-seeding. A sheet fabricated of FCSCs was also used for the differentiation assay. The results of the in vitro studies were evaluated by immunocytochemistry and Western blots for corneal epithelial cell markers (CK3/12 and Pax6) and limbal epithelial st

SUBMITTER: Park IS 

PROVIDER: S-EPMC7862470 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature