Chronic Hyperglycemia Drives Functional Impairment of Lymphocytes in Diabetic INS^C94Y Transgenic Pigs.

ABSTRACT: People with diabetes mellitus have an increased risk for infections, however, there is still a critical gap in precise knowledge about altered immune mechanisms in this disease. Since diabetic INS^C94Y transgenic pigs exhibit elevated blood glucose and a stable diabetic phenotype soon after birth, they provide a favorable model to explore functional alterations of immune cells in an early stage of diabetes mellitus in vivo. Hence, we investigated peripheral blood mononuclear cells (PBMC) of these diabetic pigs compared to non-diabetic wild-type littermates. We found a 5-fold decreased proliferative response of T cells in INS^C94Y tg pigs to polyclonal T cell mitogen phytohemagglutinin (PHA). Using label-free LC-MS/MS, a total of 3,487 proteins were

SUBMITTER: Giese IM 

PROVIDER: S-EPMC7862560 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature