Drosophila oocyte proteome composition covaries with female mating status.

ABSTRACT: Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. Here, we investigated whether mating influences mature oocyte composition in Drosophila melanogaster using a quantitative proteomic approach. Our analyses robustly identified 4,485 oocyte proteins and revealed that stage-14 oocytes from mated females differed significantly in protein composition relative to oocytes from unmated females. Proteins forming a highly interconnected network enriched for translational machinery and transmembrane proteins were increased in oocytes from mated females, including calcium bin

SUBMITTER: McDonough-Goldstein CE 

PROVIDER: S-EPMC7862673 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature