Semi-Automated Cell and Tissue Analyses Reveal Regionally Specific Morphological Alterations of Immune and Neural Cells in a Porcine Middle Cerebral Artery Occlusion Model of Stroke.

ABSTRACT: Histopathological analysis of cellular changes in the stroked brain provides critical information pertaining to inflammation, cell death, glial scarring, and other dynamic injury and recovery responses. However, commonly used manual approaches are hindered by limitations in speed, accuracy, bias, and the breadth of morphological information that can be obtained. Here, a semi-automated high-content imaging (HCI) and CellProfiler histological analysis method was developed and used in a Yucatan miniature pig permanent middle cerebral artery occlusion (pMCAO) model of ischemic stroke to overcome these limitations. Evaluation of 19 morphological parameters in IBA1⁺ microglia/macrophages, GFAP⁺ astrocytes, NeuN⁺ neuronal, FactorVIII⁺ vascular endotheli

SUBMITTER: Spellicy SE 

PROVIDER: S-EPMC7862775 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature