Targeted enrichment of novel chloroplast-based probes reveals a large-scale phylogeny of 412 bamboos.

ABSTRACT:

Background

The subfamily Bambusoideae belongs to the grass family Poaceae and has significant roles in culture, economy, and ecology. However, the phylogenetic relationships based on large-scale chloroplast genomes (CpGenomes) were elusive. Moreover, most of the chloroplast DNA sequencing methods cannot meet the requirements of large-scale CpGenome sequencing, which greatly limits and impedes the in-depth research of plant genetics and evolution.

Results

To develop a set of bamboo probes, we used 99 high-quality CpGenomes with 6 bamboo CpGenomes as representative species for the probe design, and assembled 15 M unique sequences as the final pan-chloroplast genome. A total of 180,519 probes for chloroplast DNA fragments were designed and synthesized by a novel hybridization-b

SUBMITTER: Wang J 

PROVIDER: S-EPMC7863319 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature