Spatiotemporal analysis of human intestinal development at single-cell resolution.

ABSTRACT: Development of the human intestine is not well understood. Here, we link single-cell RNA sequencing and spatial transcriptomics to characterize intestinal morphogenesis through time. We identify 101 cell states including epithelial and mesenchymal progenitor populations and programs linked to key morphogenetic milestones. We describe principles of crypt-villus axis formation; neural, vascular, mesenchymal morphogenesis, and immune population of the developing gut. We identify the differentiation hierarchies of developing fibroblast and myofibroblast subtypes and describe diverse functions for these including as vascular niche cells. We pinpoint the origins of Peyer's patches and gut-associated lymphoid tissue (GALT) and describe location-specific immune programs. We use our resource to pre

SUBMITTER: Fawkner-Corbett D 

PROVIDER: S-EPMC7864098 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature