Cryo-EM model validation recommendations based on outcomes of the 2019 EMDataResource challenge.

ABSTRACT: This paper describes outcomes of the 2019 Cryo-EM Model Challenge. The goals were to (1) assess the quality of models that can be produced from cryogenic electron microscopy (cryo-EM) maps using current modeling software, (2) evaluate reproducibility of modeling results from different software developers and users and (3) compare performance of current metrics used for model evaluation, particularly Fit-to-Map metrics, with focus on near-atomic resolution. Our findings demonstrate the relatively high accuracy and reproducibility of cryo-EM models derived by 13 participating teams from four benchmark maps, including three forming a resolution series (1.8 to 3.1 Å). The results permit specific recommendations to be made about validating near-atomic cryo-EM structures both in the context of i

SUBMITTER: Lawson CL 

PROVIDER: S-EPMC7864804 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature