Structural and Functional Characterization of a Na_v1.5-Mitochondrial Couplon.

ABSTRACT:

Rationale

The cardiac sodium channel Na_V1.5 has a fundamental role in excitability and conduction. Previous studies have shown that sodium channels cluster together in specific cellular subdomains. Their association with intracellular organelles in defined regions of the myocytes, and the functional consequences of that association, remain to be defined.

Objective

To characterize a subcellular domain formed by sodium channel clusters in the crest region of the myocytes and the subjacent subsarcolemmal mitochondria.

Methods and results

Through a combination of imaging approaches including super-resolution microscopy and electron microscopy we identified, in adult cardiac myocytes, a Na_V1.5 subpopulation in close proximity to subjacent subsarcolemm

SUBMITTER: Perez-Hernandez M 

PROVIDER: S-EPMC7864872 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature