Quantifying the effects of antibiotic treatment on the extracellular polymer network of antimicrobial resistant and sensitive biofilms using multiple particle tracking.

ABSTRACT: Novel therapeutics designed to target the polymeric matrix of biofilms requires innovative techniques to accurately assess their efficacy. Here, multiple particle tracking (MPT) was developed to characterize the physical and mechanical properties of antimicrobial resistant (AMR) bacterial biofilms and to quantify the effects of antibiotic treatment. Studies employed nanoparticles (NPs) of varying charge and size (40-500 nm) in Pseudomonas aeruginosa PAO1 and methicillin-resistant Staphylococcus aureus (MRSA) biofilms and also in polymyxin B (PMB) treated Escherichia coli biofilms of PMB-sensitive (PMB^Sens) IR57 and PMB-resistant (PMB^R) PN47 strains. NP size-dependent and strain-related differences in the diffusion coefficient values of biofilms were evident between PA

SUBMITTER: Powell LC 

PROVIDER: S-EPMC7864955 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature