A Reliable, Label Free Quality Control Method for the Production of DNA Microarrays with Clinical Applications.

ABSTRACT: The manufacture of a very high-quality microarray support is essential for the adoption of this assay format in clinical routine. In fact, poorly surface-bound probes can affect the diagnostic sensitivity or, in worst cases, lead to false negative results. Here we report on a reliable and easy quality control method for the evaluation of spotted probe properties in a microarray test, based on the Interferometric Reflectance Imaging Sensor (IRIS) system, a high-resolution label free technique able to evaluate the variation of the mass bound to a surface. In particular, we demonstrated that the IRIS analysis of microarray chips immediately after probe immobilization can detect the absence of probes, which recognizably causes a lack of signal when performing a test, with clinical relevance, u

SUBMITTER: Chiodi E 

PROVIDER: S-EPMC7865641 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature