Design of RGDS Peptide-Immobilized Self-Assembling β-Strand Peptide from Barnacle Protein.

ABSTRACT: We designed three types of RGD-containing barnacle adhesive proteins using self-assembling peptides. In the present study, three types of RGD-containing peptides were synthesized by solid-phase peptide synthesis, and the secondary structures of these peptides were analyzed by CD and FT-IR spectroscopy. The mechanical properties of peptide hydrogels were characterized by a rheometer. We discuss the correlation between the peptide conformation, and cell attachment and cell spreading activity from the viewpoint of developing effective tissue engineering scaffolds. We created a peptide-coated cell culture substrate by coating peptides on a polystyrene plate. They significantly facilitated cell adhesion and spreading compared to a non-coated substrate. When the RGDS sequence was modified at N-

SUBMITTER: Fujii D 

PROVIDER: S-EPMC7866236 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature