KV1.5-KVβ1.3 Recycling Is PKC-Dependent.

ABSTRACT: KV1.5 channel function is modified by different regulatory subunits. KVβ1.3 subunits assemble with KV1.5 channels and induce a fast and incomplete inactivation. Inhibition of PKC abolishes the KVβ1.3-induced fast inactivation, decreases the amplitude of the current KV1.5-KVβ1.3 and modifies their pharmacology likely due to changes in the traffic of KV1.5-KVβ1.3 channels in a PKC-dependent manner. In order to analyze this hypothesis, HEK293 cells were transfected with KV1.5-KVβ1.3 channels, and currents were recorded by whole-cell configuration of the patch-clamp technique. The presence of KV1.5 in the membrane was analyzed by biotinylation techniques, live cell imaging and confocal microscopy approaches. PKC inhibition resulted in a decrease of 33 ± 7% of channels in the cell surface due t

SUBMITTER: Macias A 

PROVIDER: S-EPMC7866247 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature