Ursolic acid induces apoptosis and anoikis in colorectal carcinoma RKO cells.

ABSTRACT:

Background

Ursolic acid (UA) is an anti-cancer herbal compound. In the present study, we observed the effects of UA on anchorage-dependent and -independent growth of human colorectal cancer (CRC) RKO cells.

Methods

RKO cells were cultured in conventional and detached condition and treated with UA. Cell viability was evaluated by CCK-8 assay. Cell cycle was analyzed by flow cytometry. Apoptosis was identified by Hoechst 33258 staining and flow cytometry analysis. Activities of caspases were measured by commercial kits. Reactive oxygen species (ROS) was recognized by DCFH-DA fluorescent staining. Anoikis was identified by EthD-1 fluorescent staining and flow cytometry analysis. Expression and phosphorylation of proteins were analyzed by western blot.

Results

UA inhibite

SUBMITTER: Zheng JL 

PROVIDER: S-EPMC7866452 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature