Novel live cell fluorescent probe for human-induced pluripotent stem cells highlights early reprogramming population.

ABSTRACT:

Background

Despite recent rapid progress in method development and biological understanding of induced pluripotent stem (iPS) cells, there has been a relative shortage of tools that monitor the early reprogramming process into human iPS cells.

Methods

We screened the in-house built fluorescent library compounds that specifically bind human iPS cells. After tertiary screening, the selected probe was analyzed for its ability to detect reprogramming cells in the time-dependent manner using high-content imaging analysis. The probe was compared with conventional dyes in different reprogramming methods, cell types, and cell culture conditions. Cell sorting was performed with the fluorescent probe to analyze the early reprogramming cells for their pluripotent characteristics and ge

SUBMITTER: Sriram S 

PROVIDER: S-EPMC7866770 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature