DIscBIO: A User-Friendly Pipeline for Biomarker Discovery in Single-Cell Transcriptomics.

ABSTRACT: The growing attention toward the benefits of single-cell RNA sequencing (scRNA-seq) is leading to a myriad of computational packages for the analysis of different aspects of scRNA-seq data. For researchers without advanced programing skills, it is very challenging to combine several packages in order to perform the desired analysis in a simple and reproducible way. Here we present DIscBIO, an open-source, multi-algorithmic pipeline for easy, efficient and reproducible analysis of cellular sub-populations at the transcriptomic level. The pipeline integrates multiple scRNA-seq packages and allows biomarker discovery with decision trees and gene enrichment analysis in a network context using single-cell sequencing read counts through clustering and differential analysis. DIscBIO is freely ava

SUBMITTER: Ghannoum S 

PROVIDER: S-EPMC7866810 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature