NMR Characterization of Angiogenin Variants and tRNA^Ala Products Impacting Aberrant Protein Oligomerization.

ABSTRACT: Protein oligomerization is key to countless physiological processes, but also to abnormal amyloid conformations implicated in over 25 mortal human diseases. Human Angiogenin (h-ANG), a ribonuclease A family member, produces RNA fragments that regulate ribosome formation, the creation of new blood vessels and stress granule function. Too little h-ANG activity leads to abnormal protein oligomerization, resulting in Amyotrophic Lateral Sclerosis (ALS) or Parkinson's disease. While a score of disease linked h-ANG mutants has been studied by X-ray diffraction, some elude crystallization. There is also a debate regarding the structure that RNA fragments adopt after cleavage by h-ANG. Here, to better understand the beginning of the process that leads to aberrant protein oligomerization, the solut

SUBMITTER: Fagagnini A 

PROVIDER: S-EPMC7867098 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature