Ordered Clusters of the Complete Oxidative Phosphorylation System in Cardiac Mitochondria.

ABSTRACT: The existence of a complete oxidative phosphorylation system (OXPHOS) supercomplex including both electron transport system and ATP synthases has long been assumed based on functional evidence. However, no structural confirmation of the docking between ATP synthase and proton pumps has been obtained. In this study, cryo-electron tomography was used to reveal the supramolecular architecture of the rat heart mitochondria cristae during ATP synthesis. Respirasome and ATP synthase structure in situ were determined using subtomogram averaging. The obtained reconstructions of the inner mitochondrial membrane demonstrated that rows of respiratory chain supercomplexes can dock with rows of ATP synthases forming oligomeric ordered clusters. These ordered clusters indicate a new type of OXPHOS struc

SUBMITTER: Nesterov S 

PROVIDER: S-EPMC7867189 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature